Meh, amateur. Didn’t check the PCR reaction on a gel first. Also didn’t add DNA ligase.
Protocol for Cloning
Submitted 10 months ago by fossilesque@mander.xyz to science_memes@mander.xyz
https://mander.xyz/pictrs/image/4d3c83c7-7c99-4491-b59a-3b435fab8979.jpeg
Comments
rustydomino@lemmy.world 10 months ago
RootBeerGuy@discuss.tchncs.de 10 months ago
Ah, you cloned the wrong resistance gene again…
I fondly remember my times cloning vectors… Not. My favourite was a multistep cloning and digesting protocol that I meticulously planned. It obviously failed numerous times. I got it right on the first try just making blunt ends and hoping it goes in correctly.
Toes@ani.social 10 months ago
Does it bother you when someone follows your protocol and it works on the first try?
ArbitraryValue@sh.itjust.works 10 months ago
I liked how there was a step in which I just discarded all the liquid I had been painstakingly working on. I knew there was an invisible precipitate left in the tube but it still felt like I was ruining everything.