Protocol for Cloning

Comments

• ArbitraryValue@sh.itjust.works ⁨9⁩ ⁨months⁩ ago

  I liked how there was a step in which I just discarded all the liquid I had been painstakingly working on. I knew there was an invisible precipitate left in the tube but it still felt like I was ruining everything.

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• rustydomino@lemmy.world ⁨9⁩ ⁨months⁩ ago

  Meh, amateur. Didn’t check the PCR reaction on a gel first. Also didn’t add DNA ligase.

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• RootBeerGuy@discuss.tchncs.de ⁨9⁩ ⁨months⁩ ago

  Ah, you cloned the wrong resistance gene again…

  I fondly remember my times cloning vectors… Not. My favourite was a multistep cloning and digesting protocol that I meticulously planned. It obviously failed numerous times. I got it right on the first try just making blunt ends and hoping it goes in correctly.

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  • Toes@ani.social ⁨9⁩ ⁨months⁩ ago

    Does it bother you when someone follows your protocol and it works on the first try?

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