All HPLC samples that i do, before putting them on column i filter them through short layer of silica to separate all the insoluble and entirely too polar crap
Additionally there’s also short guard column before main column, so if something goes really fucking wrong it’s the 2cm guard column that is to be replaced and not the 20cm main one
I work mostly with NP-HPLC so your situation might be a bit different, but the general principle is there
Binette@lemmy.ml 2 years ago
Idk what pretreatment really entails, but we did dilute it, if that counts 😅
skillissuer@discuss.tchncs.de 2 years ago
All HPLC samples that i do, before putting them on column i filter them through short layer of silica to separate all the insoluble and entirely too polar crap
Additionally there’s also short guard column before main column, so if something goes really fucking wrong it’s the 2cm guard column that is to be replaced and not the 20cm main one
I work mostly with NP-HPLC so your situation might be a bit different, but the general principle is there