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Binette@lemmy.ml ⁨4⁩ ⁨weeks⁩ ago

We unclogged the column, but since it was clogging it up for each sample and it’s quite damaging to the column, we decided to stop. Our budget was quite tight, since this was a school project.

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skillissuer@discuss.tchncs.de ⁨4⁩ ⁨weeks⁩ ago
jeez dude are you using some pretreatment

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- Binette@lemmy.ml ⁨4⁩ ⁨weeks⁩ ago
  Idk what pretreatment really entails, but we did dilute it, if that counts 😅
  
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  - skillissuer@discuss.tchncs.de ⁨4⁩ ⁨weeks⁩ ago
    All HPLC samples that i do, before putting them on column i filter them through short layer of silica to separate all the insoluble and entirely too polar crap
    
    Additionally there’s also short guard column before main column, so if something goes really fucking wrong it’s the 2cm guard column that is to be replaced and not the 20cm main one
    
    I work mostly with NP-HPLC so your situation might be a bit different, but the general principle is there
    
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