Comment on That's some mighty fine lanes you got there!
DrBob@lemmy.ca 1 week ago
OP doesn’t know what a good Western look like. You could surf on those waves between lanes. And the wells were overloaded - just look at the fat, blurry bands.
Comment on That's some mighty fine lanes you got there!
DrBob@lemmy.ca 1 week ago
OP doesn’t know what a good Western look like. You could surf on those waves between lanes. And the wells were overloaded - just look at the fat, blurry bands.
Contramuffin@lemmy.world 1 week ago
That’s just how ERK looks like. It’s 44 kDa and 42 kDa, which makes it so that you either see 2 bands, or, more likely, you get 1 big fuzzy band
DrBob@lemmy.ca 6 days ago
I used to do ERK! Back when half the literature referred to it as MAPK which confused all the microtubule associated protein people.
The fat caterpillar band is exactly what you see when you’ve overloaded the gel. You should drop the load and pull on an appropriate gel to able to see the gap between the 44 and 42 kd species. You may have an effect on just one.
The other issue is that if the lanes have that much protein your controls are all at ceiling. The effect could be due to variable loading but you can’t tell because one lane is at 10x ceiling and the next is at 2x ceiling. The controls look equal but you produce an effect on the target lanes. This isn’t even lab meeting quality. This is promising at the end of a shitty day quality.